RabbitAnti-IKBalpha
Cat.Number:DM-1287RQuantitysize:50ulConcentration:1mg/mlBuffer=0.01MTBS(pH7.4)with1%BSA,0.03%Proclin300and50%Glycerol.Background:ThreemajorformsofIKBlikemoleculeshavebeenidentifiedandeachischaracterisedbymultiplecopiesofankyrinrepeats.IKBalphaandIKBbetaappeartobethemajorregulatoryformsofIKBinmostcells.Theseproteinsinteractwithp65orcRelcontainingformsofNFkBandblocknuclearimportbymaskingthenuclearlocalisationsequencesofNFkB.TheactivationofNFkBinvolvestheinduciblephosphorylationandsubsequentdegradationofIKB.ImmunoblottingeasilydetectsthehyperphosphorylatedformsofIKBalpha,butnotphosphorylatedIKBbeta.Interestingly,IKBalphaandIKBbetamediatedifferentNFkBresponses.IkBalphaappearstocontrolmoretransientactivationofNFkBinresponsetoaninducer,whileIKBbetacontrolsapersistentresponse.Bcl3interactswithp50andp52containingformsofNFkB,butratherthanbeinganinhibitoritappearstofunctiontostimulatetranscription.ThedegradationofIKBisconfirmedbyimmunoblotting.Alsoknownas:NFKBIA;InhibitorofKBalpha;IkappaBalpha;I(Kappa)B(alpha);IkappaBalpha;IKBA;IKBalpha;MAD3;MAD3;MajorhistocompatibilitycomplexenhancerbindingproteinMAD3;NFkappaBinhibitoralpha;NFKBI;NFKBIA;NuclearfactorofkappalightchaingeneenhancerinBcells;NuclearfactorofkappalightpolypeptidegeneenhancerinBcellsinhibitoralpha;IKBA_HUMAN.Specificity:
●●●●RabbitPolyclonalIgG,affinitypurifiedbyProteinA.Reactswith:Human,Mouse,Rat,.Immunogen:KLHconjugatedsyntheticpeptidederivedfromhumanIKBalpha.PredictedMolecularWeight:36kDa.Storage:Shippedat4℃,Storeat-20℃(Avoidrepeatedfreeze/thawcycles).Application:WB=1:500-2000ELISA=1:500-1000IHC-P=1:400-800IHC-F=1:100-500Flow-Cyt=1μg/TestIF=1:100-500Notyettestedinotherapplications.Optimalworkingdilutionsmustbedeterminedbytheenduser.ImportantNote:Thisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplications.上海笃玛生物科技有限公司